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sdaAB [2019-07-01 10:30:56]
function
serine utilization
product
L-serine deaminase (beta chain)
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.7|Utilization of alanine/ serine]Gene
Coordinates
1,658,242 → 1,658,904
The protein
Catalyzed reaction/ biological activity
L-serine = pyruvate + NH3 (according to Swiss-Prot)Protein family
ACT domain (according to Swiss-Prot)[SW|Domains]
[SW|ACT domain] (aa 148 ... 220) (according to the Interpro database)[SW|Cofactors]
Fe-S cluster [pubmed|29292548]Expression and Regulation
Operons
genes
[gene|BC19D5B3764503298ED7136FC9F63A68F6AC464F|sdaAB]-[gene|8361D87307D2E8350945F592ABD24E2109E2E81A|sdaAA]-[gene|24B1C2A663A5B7C45940272595868F9F05A04A7B|recG]
description
[Pubmed|22383849]
view in new tabBiological materials
Mutant
BKE15850 (Δ[gene|BC19D5B3764503298ED7136FC9F63A68F6AC464F|sdaAB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTATTCCTCCTTATGA, downstream forward: _UP4_TAGCGAAAAGGGTCAGGAGGBKK15850 (Δ[gene|BC19D5B3764503298ED7136FC9F63A68F6AC464F|sdaAB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTATTCCTCCTTATGA, downstream forward: _UP4_TAGCGAAAAGGGTCAGGAGGlacZ fusion
pGP2275 (in [SW|pAC5]) (GP2967), available in [SW|Jörg Stülke]'s labReferences
22383849,22686449,24161940